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KMID : 0613820090190010152
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Journal of Life Science
2009 Volume.19 No. 1 p.152 ~ p.155
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A Gene-based dCAPS Marker for Selecting old-gold-crimson () Fruit Color Mutation in Tomato
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Park Young-Hoon
Lee Yong-Jae
Kang Jum-Soon
Choi Young-Whan
Son Beung-Gu
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Abstract
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The old-gold-crimson () fruit color mutation produces deep red tomato fruit with high lycopene content. age is a null mutation allele of lycopene--cyclase (Crt-b) gene (B locus) that converts lycopene to -carotene in the cartenoid biosynthesis pathway in tomato. Breeding of high lycopene tomato cultivars can be accelerated by marker-assisted selection (MAS) for introgression of allele by using a gene-based DNA marker. In order to develop a marker, single nucleotide deletion of adenine(A) with. in a poly-A repeat that has been known to be responsible for frame-shift mutation of was confirmed by resequencing mutant allele and wild-type allele at B locus of several tomato lines. For allele discrimination and detection of , derived CAPS (dCAPS) approach was used by designing a primer that artificially introduced restriction enzyme recognition site of Hin fI in PCR products from allele. This dCAPS marker is co-dominant gene-based PCR marker that can be efficiently used for MAS breeding program aiming the development of high lycopene tomato.
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KEYWORD
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Tomato, derived CAPS (dCAPS), marker-assisted selection (MAS), lycopene, old-gold-crimson ()
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